pebmulti-neo vector (FUJIFILM)
90
Structured Review
FUJIFILM
pebmulti-neo vector
Pebmulti Neo Vector, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pebmulti-neo+vector/pebmulti+neo+vector/bio_rxiv__2025__01__06__631603-143-7-9
Average 90 stars, based on 1 article reviews
Pebmulti Neo Vector, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pebmulti-neo+vector/pebmulti+neo+vector/bio_rxiv__2025__01__06__631603-143-7-9
Average 90 stars, based on 1 article reviews
pebmulti-neo vector - by Bioz Stars,
2026-09
90/100 stars
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Plasmid Preparation:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a Sequencing:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a Clone Assay:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a Produced:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a Derivative Assay:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a Luciferase:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a Transfection:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a Stable Transfection:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a Expressing:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a Selection:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a Polymerase Chain Reaction:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a Modification:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a Amplification:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a cDNA Library Assay:Article Title: Structural basis of trans -synaptic interactions between PTPδ and SALMs for inducing synapse formation Article Snippet: The cDNAs encoding mouse PTPδ Ig1–Ig3 (residues 28–325) and PTPδ Ig1–Fn1 (residues 28–418; A9B+, A9B– or A–B+) were cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries) with the N-terminal signal sequence derived from pHLsec vector . Article Title: PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer. Article Snippet: Three fragments were joined using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs, MA, USA) according to the manufacturer’s protocol, and inserted into the multiple cloning site (MCS) of the Article Title: Structural insights into heterohexameric assembly of epilepsy-related ligand–receptor complex LGI1–ADAM22 Article Snippet: The vectors for the co-expression were the pEBMulti-Neo vector ( Article Title: Evidence for a functional role of Start , a long noncoding RNA, in mouse spermatocytes Article Snippet: The thymidine kinase (TK) promoter was obtained as a 750-bp fragment from a Article Title: C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins Article Snippet: 1Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA 2Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA 3Laboratory of Neural Connectivity, Brain Research Institute, Faculties of Medicine and Science, University of Zürich, Zürich, Switzerland 4Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA 5Department of Neuroscience, The University of Texas at Austin, Austin, TX, USA 6The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA Article Title: Structural insights into modulation and selectivity of transsynaptic neurexin–LRRTM interaction Article Snippet: The genes encoding human LRRTM2 LRR (residues 1–371), LRRTM3 LRR (residues 1–371), and LRRTM4 LRR (residues 1–370) with the C-terminal His 6 tag were then cloned into the pEBMulti-Neo vector (Wako Pure Chemical Industries). Article Title: The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata Article Snippet: The GloSensor-22F cAMP gene (Promega Corporation, Fitchburg, WI, USA), a luciferase-based cAMP reporter, was cloned into a |